APA
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Sheikh, M. O., Halmo, S. M., Patel, S., Middleton, D., Takeuchi, H., Schafer, C. M., … Wells, L. (2017). Rapid screening of sugar-nucleotide donor specificities of putative glycosyltransferases. Glycobiology.
Chicago/Turabian
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Sheikh, M. Osman, Stephanie M. Halmo, Sneha Patel, Dustin Middleton, Hideyuki Takeuchi, Christopher M. Schafer, Christopher M. West, et al. “Rapid Screening of Sugar-Nucleotide Donor Specificities of Putative Glycosyltransferases.” Glycobiology (2017).
MLA
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Sheikh, M. Osman, et al. “Rapid Screening of Sugar-Nucleotide Donor Specificities of Putative Glycosyltransferases.” Glycobiology, 2017.
BibTeX Click to copy
@article{m2017a,
title = {Rapid screening of sugar-nucleotide donor specificities of putative glycosyltransferases},
year = {2017},
journal = {Glycobiology},
author = {Sheikh, M. Osman and Halmo, Stephanie M. and Patel, Sneha and Middleton, Dustin and Takeuchi, Hideyuki and Schafer, Christopher M. and West, Christopher M. and Haltiwanger, Robert S. and Avci, Fikri Y. and Moremen, Kelley W. and Wells, Lance}
}
Determining the correct enzymatic activity of putative glycosyltransferases (GTs) can be challenging as these enzymes can utilize multiple donor and acceptor substrates. Upon initial determination of the donor-sugar nucleotide(s), a GT utilizes various acceptor molecules that can then be tested. Here, we describe a quick method to screen sugar-nucleotide donor specificities of GTs utilizing a sensitive, nonradioactive, commercially available bioluminescent uridine diphosphate detection kit. This in vitro method allowed us to validate the sugar-nucleotide donor-substrate specificities of recombinantly expressed human, bovine, bacterial and protozoan GTs. Our approach, which is less time consuming than many traditional assays that utilize radiolabeled sugars and chromatographic separations, should facilitate discovery of novel GTs that participate in diverse biological processes.